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What are the methods of gene cloning ?
I am in a confusion about which are the exact methods of gene cloning.. plz help me to sort them out
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Gene splicing is a natural phenomenon that occurs within the cell nucleus (and renders different versions of the same mRNA).
Gene cloning is an artificial technique to make copies of a DNA sequence previously isolated. It is very long to explain to someone who doesn't understand the basics of DNA and protein expression so I would advice you to read about it in real science books about genetics or molecular and cell biology.
Here are a few but the best I've read:
Molecular Biology of the Cell (Bruce Alberts et al)
Genes (Lewin)