There are so many reasons you can miss a period other than just pregnancy. I noticed in one of the comments you noted you have several children already, so you likely have a gut feeling as to whether you are pregnant or not already. I know several people with multiple children and they say by their second or third they know within a couple weeks and don't even need a test - the can feel it almost down to the day. This could be the case for you.
Other reasons to miss period include:
*Stress (as others have mentioned)
*Being underweight or overweight - girls often need to be around 100 pounds, and their period may stop if they dip below that weight.
*Having too small of a calorie intake, or too low body fat percentage
*A sudden change in diet (becoming vegetarian, for example)
*Major injuries
*Sudden change in daily activity (suddenly starting exercise)
*Regular high levels of activity (usually only applies to serious athletes - your body senses it's priorities are not to bear children and it no longer need it's period)
*Starting new medications
*Plan B or other hormone pills (notorious for destroying your normal cycle)
Also, things like cysts can stop your cycle. If you've had a child within the last year it can take quite some time to become regular again.

If you really do think you are pregnant, give it a couple weeks and test again. It's possible your levels are just not high enough to trip the test, or it's possible there is an underlying medical reason like a very early miscarriage.
If you think you are pregnant and don't want to wait, head to the doctor for a blood test (something I'm sure you've done before). No point getting a urine test at the doctors, to be honest. The use standard drug store tests as well, and not even ones that detect the lowest level. I know here, the tests doctors give are the same as the ones you get from the dollar store, so a blood test is much better.
If you wait it out a month, your fairly sure your not pregnant, (no positive tests) and you see no other reasons, and miss your next, its time for a trip to your doctor. It's most likely nothing and your body has been thrown off cycle by something, but it could also be something more serious.
Also, hard to tell your age by your picture, but women are reaching menopause much earlier than normal these days - sometimes in their 30's or 40's, and it's not uncommon to see cases as early as a women's 20's. It's possible your just starting menopause, in which case a doctors trip again is in order - unless your very against medication, there are many medications you may consider taking. I only mention this as I lived with my mother while she went through menopause - she was against medication and she made life absolutely miserable for everyone and was extremely emotional. It's still not over and it's going on 8 years - really should be corrected. She bursts into tears for things like burning a batch of cookies or missing a spot when washing dishes, and it's not something you should suffer with. Sorry if I'm grossly overestimating your age, but as I said, menopause can hit very early these days.

Here is a very good page from WebMD which is a great and reliable source for health information.
Here is another WebMD page, though this one is discussion on the topic which may lead to some insight by other people that have had the same issue.
And finally, here is a little more from babymed.com

Long story shot, you could be pregnant but you'd likely be able to tell. Most likely it's just a fluke (something like chemical pregnancy) or a lifestyle change, but it can also be more serious medical conditions, so If it's worrying you, head to the doctor.

Good luck!
Source(s):
http://women.webmd.com/tc/missed-or-irregular-periods-topic-overview
http://blogs.webmd.com/womens-health/2007/06/missed-your-period-but-youre-n...
http://babymed.com/faq/Content.aspx?14361


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