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 M¢25  Funded By Mahalo ? |  July 18, 2009 06:36 AM

how is tamiflu resistance tested (to identify flu resistant to tamiflu treatment)?

this has been in the news, but i haven't seen anything explaining how resistance is confirmed. connected with this, but entirely optional to an answer: (1) how long does it take to test one specimen for resistance? (2) when resistance was found in the news-reported cases, was it probably true that all of the flu virus in the person's body was resistant, or only a subset? (3) has loss of resistance--e.g., after a particular drug stops being widely used--ever been documented (probably in a lab, and not from people)?
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gamchep gamchep
 
 August 16, 2009 05:07 PM
98 percent of this year's circulating H1N1 flu strains are immune to Tamiflu, compared with only 12 percent during the 2007-2008 flu season says an article in Scientific American in March 2009. Using a modified amplification refractory mutation system PCR (ARMS-PCR), you can simultaneously detect wild-type or mutant virus with the 274 mutation in a single PCR. It is a very specialised test and not widely available
Regards
Gamchek


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mysterygir... mysterygir...
 
August 17, 2009 03:23 AM
Great Answer!

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gno gno
 
August 17, 2009 12:52 PM
Nicely done, @gamchep, and welcome to Mahalo!

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ghanan2000... ghanan2000...
 
August 18, 2009 04:33 PM
Nice Job !

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