There were 50, including the "president" John Hancock from the 13 colonies:

President of Congress
1. John Hancock (Massachusetts)

New Hampshire
2. Josiah Bartlett
3. William Whipple
4. Matthew Thornton

Massachusetts
5. Samuel Adams
6. John Adams
7. Robert Treat Paine
8. Elbridge Gerry

Rhode Island
9. Stephen Hopkins
10. William Ellery

Connecticut
11. Roger Sherman
12. Samuel Huntington
13. William Williams
14. Oliver Wolcott

New York
15. William Floyd
16. Philip Livingston
17. Francis Lewis
18. Lewis Morris

New Jersey
19. Richard Stockton
20. John Witherspoon
21. Francis Hopkinson
22. John Hart
23. Abraham Clark

Pennsylvania
24. Robert Morris
25. Benjamin Rush
26. Benjamin Franklin
27. John Morton
28. George Clymer
29. James Smith
30. George Taylor
31. James Wilson
32. George Ross

Delaware
33. George Read
34. Caesar Rodney
35. Thomas McKean

Maryland
36. Samuel Chase
37. William Paca
38. Thomas Stone
39. Charles Carroll of Carrollton

Virginia
40. George Wythe
41. Richard Henry Lee
42. Thomas Jefferson
43. Benjamin Harrison
44. Thomas Nelson, Jr.
45. Francis Lightfoot Lee
46. Carter Braxton

North Carolina
47. William Hooper
48. Joseph Hewes
49. John Penn

South Carolina
50. Edward Rutledge
51. Thomas Heyward, Jr.
52. Thomas Lynch, Jr.
53. Arthur Middleton

Georgia
54. Button Gwinnett
55. Lyman Hall
56. George Walton

There were also 8 who were thought to be in attendance for the signing but never did: John Alsop, George Clinton, John Dickinson, Charles Humphreys, Robert R. Livingston, John Rogers, Thomas Willing, and Henry Wisner.

http://en.wikipedia.org/wiki/United_States_Declaration_of_Independence
Source(s):
http://en.wikipedia.org/wiki/United_States_Declaration_of_Independence

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