If you're referring to a virtual CD image (.iso, .cue, etc), you should be able to just delete those like you would any regular file. Just be sure to unmount it first using the virtual CD software (i.e. DaemonTools) before deleting, otherwise your PC will tell you that the file's in use.

On the other hand, if your USB drive has a virtual partition, you can just format your drive on any computer. That will wipe all the data (including the partitions) on the drive and give you just one big chunk of free space.

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