Quiznos sandwich franchise chain launched a controversial advertising campaign in May 2009, entitled, 2 Girls, 1 Sub. Playboy teamed up with the sandwich shop franchise to create a commercial based on the Internet meme, 2 Girls, 1 Cup. The meme features two women acting out several sexual fetishes, including defecating into a cup. Advertising insiders have questioned Quinzos' marketing logic in associating the sandwiches with eating human excrement.Mediabistro: Playboy + Quizno's = 2 Girls 1 Sub (May 19, 2009)

Quiznos is a sandwich restaurant franchise. The first Quiznos Sub restaurant opened in Denver, Colorado in 1981. Rick Schaden opened the first franchise in Boulder, Colorado in 1987. In 1991, Schaden and his father, Dick Schaden, purchased the 18-restaurant franchise operation. Quiznos celebrated its 25 anniversary in 2006.Official Site: About Quiznos

The Internet meme, 2 Girls, 1 Cup was originally a trailer for the fetish film, Hungry Bitches, produced by a Brazilian company, MFX-Video. The video clip featuring two women acting out various sexual fetishes became an Internet sensation in 2007. Marco Fiorito, founder of MFX, claimed that the scenes involving excrement was actually filmed using chocolate.

2 Girls, 1 Cup was declared obscene by the United States government. Danilo Croce, who was allegedly involved in distributing the video was charged with conspiracy to distribute obscene material.Smoking Gun: 2 Girls, 1 Cup: The Real Poop (November 30, 2007)